Culture Medium BT (for marine algae, except diatoms)
Final concentrations of additions and preparation of stock solutions:
|
final concentration |
Trace elements |
Mol L-1 |
Na2EDTA |
1x10-4 |
Fe-(III)-citrate |
2,5x10-6 |
MnCl2x4H2O |
5x10-7 |
ZnSO4x7H2O |
5x10-8 |
Na2MoO4x2H2O |
2,5x10-9 |
CoCl2x6H2O |
5x10-9 |
CuSo4x5H2O |
5x10-10 |
H2SeO3 |
5x10-10 |
NaVO3 |
5x10-10 |
NiSO4x6H2O |
5x10-10 |
|
|
Nutrients |
|
Na NO3 |
4,42x10-4 |
Na2glyceroPO4 |
5x10-5 |
NaH2PO4 |
1,81x10-5 |
NH4Cl |
2,5x10-5 |
|
|
Buffer |
|
TRIS pH 7,2 |
1x10-3 |
|
|
Vitamins |
|
Thiamine |
3x10-7 |
Biotin |
2,1x10-9 |
Vitamin B12 |
3,7x10-10 |
Preparation of stock solutions: